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KMID : 0895420140240040416
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Journal of Korean Society of Occupational and Enviromental Hygiene
2014 Volume.24 No. 4 p.416 ~ p.424
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Identification of Predominant Bacteria and Fungi in the Industry Treating Soluble Metal Working Fluids
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Pakr Hae-Dong
Park Dong-Jin
Park Hyun-Hee
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Abstract
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Objectives: The objective of this study is to analyze the predominant microorganisms in the industry treating MWFs(Metal working fluids).
Methods: The bacteria and fungi were collected by agar plate impaction and bulk MWFs in storage tank at 54 sites in 9 shops in South Korea. The dominant bacteria and fungi isolated from agar media were identified by fatty acid analysis and morphological analysis, respectively.
Results: Totally 111 dominant bacteria were identified in the process, outdoor, and bulk MWFs. The predominant bacterial genus was Micrococcus and Bacillus in the process and outdoor, Pseudomonas in bulk MWF. Among the identified 119 strains of fungi, Cladosporium and Penicillium genus were dominated. The ratios of bacteria designated biosafety level 2 and 1 were 30% and 21%, respectively.
Conclusions: This study has investigated the dominant microorganisms in soluble MWF using industry. And it was useful that the qualitative evaluation method along with quantitative analysis for better understanding of the biological factors in the work environment.
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KEYWORD
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biological agent, bacteria, fungi, microbial identification, metal working fluids
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